9 CFR § 113.32 - Detection of Brucella contamination.

---
identifier: "/us/cfr/t9/s113.32"
source: "ecfr"
legal_status: "authoritative_unofficial"
title: "9 CFR § 113.32 - Detection of Brucella contamination."
title_number: 9
title_name: "Animals and Animal Products"
section_number: "113.32"
section_name: "Detection of Brucella contamination."
chapter_name: "ANIMAL AND PLANT HEALTH INSPECTION SERVICE, DEPARTMENT OF AGRICULTURE"
subchapter_number: "E"
subchapter_name: "VIRUSES, SERUMS, TOXINS, AND ANALOGOUS PRODUCTS; ORGANISMS AND VECTORS"
part_number: "113"
part_name: "STANDARD REQUIREMENTS"
positive_law: false
currency: "2026-04-05"
last_updated: "2026-04-05"
format_version: "1.1.0"
generator: "[email protected]"
authority: "21 U.S.C. 151-159; 7 CFR 2.22, 2.80, and 371.4."
regulatory_source: "34 FR 18004, Nov. 7, 1969, unless otherwise noted."
cfr_part: "113"
---


# 113.32 Detection of Brucella contamination.

The test for detection of Brucella contamination provided in this section shall be conducted when such a test is prescribed in an applicable Standard Requirement or in a filed Outline of Production for the product.

(a) One ml of the minced tissue used as the source of cells or 1 ml of the extract of the tissue prior to the addition of antibiotics, diluent and stabilizer, shall be inoculated onto each of three tryptose agar plates and incubated in a 10 percent CO<sub>2</sub> atmosphere at a temperature of 35-37 °C for at least 7 days.

(b) If colonies are identified as Brucella, the biological product is unsatisfactory.

(c) If colonies suspicious of Brucella are observed but cannot be identified as a Brucella species, either

(1) The biological product shall be regarded as unsatisfactory and destroyed; or

(2) Further subculture or other procedures shall be carried out until a positive identification can be made.

[38 FR 29888, Oct. 30, 1973]