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21 CFR § 112.145 - What requirements apply to testing the environment for Listeria species or L. monocytogenes?

---
identifier: "/us/cfr/t21/s112.145"
source: "ecfr"
legal_status: "authoritative_unofficial"
title: "21 CFR § 112.145 - What requirements apply to testing the environment for Listeria species or L. monocytogenes?"
title_number: 21
title_name: "Food and Drugs"
section_number: "112.145"
section_name: "What requirements apply to testing the environment for Listeria species or L. monocytogenes?"
chapter_name: "FOOD AND DRUG ADMINISTRATION, DEPARTMENT OF HEALTH AND HUMAN SERVICES"
subchapter_number: "B"
subchapter_name: "FOOD FOR HUMAN CONSUMPTION"
part_number: "112"
part_name: "STANDARDS FOR THE GROWING, HARVESTING, PACKING, AND HOLDING OF PRODUCE FOR HUMAN CONSUMPTION"
positive_law: false
currency: "2026-04-05"
last_updated: "2026-04-05"
format_version: "1.1.0"
generator: "[email protected]"
authority: "21 U.S.C. 321, 331, 342, 350h, 371; 42 U.S.C. 243, 264, 271."
regulatory_source: "80 FR 74547, Nov. 27, 2015, unless otherwise noted."
cfr_part: "112"
---

# 112.145 What requirements apply to testing the environment for Listeria species or L. monocytogenes?

All of the following testing requirements apply for the growing, harvesting, packing, and holding environment for *Listeria* species or *L. monocytogenes.*

(a) You must establish and implement a written environmental monitoring plan that is designed to identify *L. monocytogenes* if it is present in the growing, harvesting, packing, or holding environment.

(b) Your written environmental monitoring plan must be directed to sampling and testing for either *Listeria* species or *L. monocytogenes.*

(c) Your written environmental monitoring plan must include a sampling plan that specifies:

(1) What you will test collected samples for (*i.e., Listeria* species or *L. monocytogenes*);

(2) How often you will collect environmental samples, which must be no less than monthly, and at what point during production you will collect the samples; and

(3) Sample collection sites; the number and location of sampling sites must be sufficient to determine whether measures are effective and must include appropriate food contact surfaces and non-food-contact surfaces of equipment, and other surfaces within the growing, harvesting, packing, and holding environment.

(d) You must aseptically collect environmental samples and test them for *Listeria* species or *L. monocytogenes* using a method as set forth in § 112.152.

(e) Your written environmental monitoring plan must include a corrective action plan that, at a minimum, requires you to take the actions in § 112.146, and details when and how you will accomplish those actions, if the growing, harvesting, packing, or holding environment tests positive for *Listeria* species or *L. monocytogenes.*