49 CFR § 655.21 - Drug testing.

---
identifier: "/us/cfr/t49/s655.21"
source: "ecfr"
legal_status: "authoritative_unofficial"
title: "49 CFR § 655.21 - Drug testing."
title_number: 49
title_name: "Transportation"
section_number: "655.21"
section_name: "Drug testing."
chapter_name: "FEDERAL TRANSIT ADMINISTRATION, DEPARTMENT OF TRANSPORTATION"
part_number: "655"
part_name: "PREVENTION OF ALCOHOL MISUSE AND PROHIBITED DRUG USE IN TRANSIT OPERATIONS"
positive_law: false
currency: "2026-03-24"
last_updated: "2026-03-24"
format_version: "1.1.0"
generator: "[email protected]"
authority: "49 U.S.C. 5331 (as amended); 49 CFR 1.91"
regulatory_source: "66 FR 42002, Aug. 9, 2001, unless otherwise noted."
cfr_part: "655"
---


# 655.21 Drug testing.

(a) An employer shall establish a program that provides testing for prohibited drugs and drug metabolites in the following circumstances: pre-employment, post-accident, reasonable suspicion, random, and return to duty/follow-up.

(b) When administering a drug test, an employer shall ensure that the following drugs are tested for:

(1) Marijuana;

(2) Cocaine;

(3) Opioids;

(4) Amphetamines; and

(5) Phencyclidine.

(c) Consumption of these products is prohibited at all times.

[66 FR 42002, Aug. 9, 2001, as amended at 84 FR 16775, Apr. 23, 2019]